English
norskBlar i forfatter "Myrmel, Truls"
Viser treff 1-18 av 18
-
The acute phase of experimental cardiogenic shock is counteracted by microcirculatory and mitochondrial adaptations
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
Adaptive fluctuation imaging captures rapid subcellular dynamics
(Journal article; Tidsskriftartikkel, 2019-07-22)In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, ... -
Adaptive fluctuation imaging captures rapid subcellular dynamics
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-22)In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, ... -
Combined Therapy With Dobutamine and Omecamtiv Mecarbil in Pigs With Ischemic Acute Heart Failure Is Attributed to the Effect of Dobutamine
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-23)Inotropic support in ischemic acute heart failure (AHF) is controversial. We tested a therapeutic principle for AHF by combining a low dose of omecamtiv mecarbil (OM; 0.25 mg/kg bolus plus 0.25 mg/kg/h) with a low dose of dobutamine (Dobut; 1.25 µg/kg/min). In 10 pigs subjected to myocardial ischemia by left coronary microembolization, this cotreatment increased cardiac power (CP) from 0.48 ± 0.14 ... -
Direct angiography demonstrates equal 8-12 years patency rates of radial artery and saphenous vein grafts
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-30)Objectives: The benefits of coronary artery bypass surgery depend on lasting graft patency. To aid rational graft selection, the relative long-term merits of radial artery and saphenous vein grafts need to be determined by a gold standard method and with minimal clinically driven selection bias. Methods: The patency rates of various conduits were determined by direct angiography in 76 patients from ... -
The effect of Riociguat on cardiovascular function and efficiency in healthy, juvenile pigs
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-12)<p><i>Introduction - </i>Riociguat is a soluble guanylate cyclase stimulator approved for the treatment of pulmonary hypertension. Its effect on cardiometabolic efficiency is unknown. A potential cardiac energy sparing effect of this drug could imply a positive prognostic effect, particularly in patients with right heart failure from pulmonary hypertension. <p><i>Method - </i>We infused Riociguat ... -
Extent of Preoperative False Lumen Thrombosis Does Not Influence Long-Term Survival in Patients With Acute Type A Aortic Dissection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)Background-—Partial thrombosis of the false lumen has been related to aortic growth, reoperations, and death in the chronic phase of type B and repaired type A aortic dissections. The impact of preoperative false lumen thrombosis has not been studied previously. We used data from a contemporary, multinational database on aortic dissections to evaluate whether different degrees of preoperative ... -
Health-related quality of life after extracorporeal membrane oxygenation: a single centre's experience
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-07)<i>Aims</i>: The aims of this study were to evaluate health-related quality of life (HRQL) among patients treated with extracorpo-real membrane oxygenation (ECMO) in northern Norway over a period of 27years (1988–2015) and to identify variables related to HRQL.<p> <p><i>Methods and results</i>: A retrospective inquiry of the ECMO registry at the University Hospital of North Norway identified 74 ... -
Hemodynamic effects of a soluble guanylate cyclase stimulator, Riociguat, and an activator, Cinaciguat, during NO-modulation in healthy pigs
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-14)Cardiovascular diseases are often characterized by dysfunctional endothelium. To compensate for the related lack of nitric oxide (NO), a class of soluble guanylate cyclase (sGC) stimulators and activators have been developed with the purpose of acting downstream of NO in the NO-sGC-cGMP cascade. These drugs have been discovered using photoaffinity labeling of sGC and high-throughput screening of a ... -
High-resolution visualization and assessment of basal and OXPHOS-induced mitophagy in H9c2 cardiomyoblasts
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-05)Mitochondria are susceptible to damage resulting from their activity as energy providers. Damaged mitochondria can cause harm to the cell and thus mitochondria are subjected to elaborate quality-control mechanisms including elimination via lysosomal degradation in a process termed mitophagy. Basal mitophagy is a house-keeping mechanism fine-tuning the number of mitochondria according to the metabolic ... -
Learning Nanoscale Motion Patterns of Vesicles in Living Cells
(Conference object; Konferansebidrag, 2020-08-05)Detecting and analyzing nanoscale motion patterns of vesicles, smaller than the microscope resolution (~250 nm), inside living biological cells is a challenging problem. State-of-the-art CV approaches based on detection, tracking, optical flow or deep learning perform poorly for this problem. We propose an integrative approach, built upon physics based simulations, nanoscopy algorithms, and shallow ... -
Learning nanoscale motion patterns of vesicles in living cells
(Chapter; Bokkapittel, 2020)Detecting and analyzing nanoscale motion patterns of vesicles, smaller than the microscope resolution (~250 nm), inside living biological cells is a challenging problem. State-of-the-art CV approaches based on detection, tracking, optical flow or deep learning perform poorly for this problem. We propose an integrative approach, built upon physics based simulations, nanoscopy algorithms, and shallow ... -
Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-11-12)Mitochondria are essential energy-providing organelles of particular importance in energy-demanding tissue such as the heart. The production of mitochondria-derived vesicles (MDVs) is a cellular mechanism by which cells ensure a healthy pool of mitochondria. These vesicles are small and fast-moving objects not easily captured by imaging. In this work, we have tested the ability of the optical ... -
Opposite diastolic effects of omecamtiv mecarbil versus dobutamine and ivabradine co-treatment in pigs with acute ischemic heart failure
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-11)Acute ischemic cardiogenic shock is associated with poor prognosis, and the impact of inotropic support on diastolic function in this context is unclear. We assessed two suggested new inotropic strategies in a clinically relevant pig model of ischemic acute heart failure (AHF): treatment with the myosin activator omecamtiv mecarbil (OM) or dobutamine and ivabradine (D+I). Left ventricular (LV) ... -
The risk factors for radial artery and saphenous vein graft occlusion are different
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-06-02)Objectives. To determine risk factors for radial artery and saphenous vein graft occlusion during long-term follow-up after coronary artery bypass grafting (CABG). Methods: From a cohort of 119 patients who had received a radial artery graft, 76 – of whom 55 also had at least one saphenous vein graft – underwent a preplanned direct angiography and anthropometric, biochemical, and endothelial ... -
Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-23)This three-dimensional structured illumination microscopy (3DSIM) dataset was generated to highlight the suitability of 3DSIM to investigate mitochondria-derived vesicles (MDVs) in H9c2 cardiomyoblasts in living or fxed cells. MDVs act as a mitochondria quality control mechanism. The cells were stably expressing the tandem-tag eGFP-mCherry-OMP25-TM (outer mitochondrial membrane) which can be used ... -
Vasopressin impairs brain, heart and kidney perfusion: an experimental study in pigs after transient myocardial ischemia
(Journal article; Tidsskriftartikkel; Peer reviewed, 2008-02-21)Introduction: Arginine vasopressin (AVP) is increasingly used to restore mean arterial pressure (MAP) in low-pressure shock states unresponsive to conventional inotropes. This is potentially deleterious since AVP is also known to reduce cardiac output by increasing vascular resistance. The effects of AVP on blood flow to vital organs and cardiac performance in a circulation altered by cardiac ... -
Virtual labeling of mitochondria in living cells using correlative imaging and physics-guided deep learning
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-28)Mitochondria play a crucial role in cellular metabolism. This paper presents a novel method to visualize mitochondria in living cells without the use of fluorescent markers. We propose a physics-guided deep learning approach for obtaining virtually labeled micrographs of mitochondria from bright-field images. We integrate a microscope’s point spread function in the learning of an adversarial neural ...
